Reference #: 1014097 SHIFT:Day (United States of America)

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We strongly encourage all candidates of diverse backgrounds and lived experiences to apply.

A Brief Overview We have an exciting opportunity for a highly motivated technician to join the Resuscitation Science Center at The Children's Hospital of Philadelphia, Philadelphia, PA, as a member and participant in our molecular lab research, with a specific focus on mitochondrial physiology and other standard molecular assay work such as Western Blots. Our research focuses on advanced therapies for treating cardiac arrest and traumatic brain injuries (TBI) along with potential therapeutics for mitochondrial diseases to reduce neurological and mitochondrial damage. Clinically, our research helps to improve pediatric survival outcomes and reduce neurological deficits post-cardiac arrest/TBI, in addition to growing the scope of knowledge related to mitochondrial disorder therapies. Our focus is on critical illness: Traumatic Brain Injury, Cardiac Arrest, Extracorporeal Life Support, Cardiopulmonary Bypass, and Toxicology. Our laboratory is truly translational laboratory that span multiple models of critical illness that range from cell culture models to small animal and large animals (swine).

The successful candidate should possess exceptional organization, communication, time management, and critical thinking skills and be willing to work in both a team and independent setting. Prior lab experience in cell/molecular or other biological studies is required. A willingness to and/or experience with working hands on with both large and small animals for experiments is preferred but not required. Due to the time sensitive nature of the experiments, the successful candidate must be willing to work flexible hours as dictated by the varying timelines of the different studies/experiments.

What you will do Prepare and aliquot common reagents used in the lab's molecular experiments Perform or assist with basic necropsy animal procedures (dissection, tissue collection, and sample collection) for further downstream analysis Perform standard molecular and cellular biology lab techniques and assays (PCR/qPCR, western blotting, ELISA assays, etc.) Perform mitochondrial specific assays such as high-resolution respirometry, microdialysis, citrate synthase activity on samples collected from animal experiments Help store and log biobanked tissue samples from studies in sample management organization software (Freezerworks) Compile and analyze data using Microsoft Office programs, and possibly other data programs including but not limited to Graphpad Prism and Matlab Design and execute experiments in a timely manner under the leadership of the Project Lead and/or PI.

Education Qualifications High School Diploma / GED Required Bachelor's Degree in a relevant field of cell/molecular or other biological studies,  Preferred

Experience Qualifications At least two (2) years of related classroom experience or successful completion of a technical t aining program (eg. Wistar, WPSI) and research laboratory experience Required At least three (3) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Preferred Prior lab experience in cell/molecular or other biological studies (e.g. neuroscience, animal science, pathology, microscopy, pre-med/pre-vet, bioengineering, or closely related fields) Preferred

Skills and Abilities Applicant must possess strong communicative skills; prioritize initiatives and projects across all stages while team building through excellent problem-solving skills. Ability to objectively critique results and present as valid or questionable, taking into account all aspects of study design, technical issues, and limitations of workflows is required. Ability to identify, troubleshoot data or state abnormalities by reviewing all major aspects of an assay is required. Ability to work in a dynamic environment and achieve deadlines while maintaining an organized workspace. Ability to work in a team environment interacting with various investigators, learners and other team members of the lab.

To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community. As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must be fully vaccinated against COVID-19 and receive an annual influenza vaccine. Learn more.

Employees may request exemptions for valid religious and medical reasons. Start dates may be delayed until candidates are immunized or exemption requests are reviewed.

EEO / VEVRAA Federal Contractor |  Tobacco Statement

Children's Hospital of Philadelphia is an equal opportunity employer. We do not discriminate on the basis of race, color, gender, gender identity, sexual orientation, age, religion, national or ethnic origin, disability or protected veteran status.