Apply for Job Job ID364415 LocationTwin Cities Job FamilyResearch-Researchers Full/Part TimePart-Time Regular/TemporaryRegular Job Code9742R5 Employee ClassAcad Prof and Admin Add to Favorite Jobs Email this Job About the Job Job duties: Responsibilities: Our lab is interested in understanding the fundamental biological mechanisms for immunological tolerance during autoimmunity and transplantation, directed at therapeutic intervention strategies. We are using autoimmune animal models to understand biological processes involved with autoimmune diabetes and multiple sclerosis. Immune checkpoint inhibition as well as chimeric antigen receptor T cells are being investigated as therapeutic options for restoring immunological tolerance. The post-holder will be involved in the design, development, execution and characterization of novel pathways as therapeutic targets. Incumbent's responsibilities will involve the management and evaluation of experimental cellular therapies for diabetes using mouse models; this will include genotyping (PCR), drug preparation and delivery and dissection of mice upon trial completion; play a role in trial evaluation via protein and RNA analysis including histology, immunohistochemistry, cell culture, cellular assays, RT-PCR, Western blot analysis, ELIspot and cellular imaging. Scientist will help use the data collected from these evaluation methods to initiate subsequent trials; assist in normal lab and mouse colony maintenance and organization, supervise other technicians and undergraduate students; and perform other duties as assigned. This position a .75fte with benefits and may increase to a 1.00fte dependent on funding and work available. Specific Duties: Perform independent research projects according to established protocols and following direction of PI to include: MAb production, cell culture, CAR Treg generation, evaluation, and immune suppression assays, drug preparation, tolerance experiments, diabetes assessment, plasmid transfection, drug preparation and administration, assist with PBMC and tissue preparations, assist with surgical procedures, and perform flow cytometry analysis, and immunohistochemistry. Prepare written and graphical descriptions of results to discuss with PI. Review scientific literature, technical documents, and perform other experiments as assigned by the PI. (53%) Participate in monitoring rodent populations by PCR, Flow Cytometry, and diabetes assessment prior to and following treatments. (7%) Dirty mouse facility experimental design, execution, monitoring, injections, and tissue harvest, and analysis to evaluate the role of microbes on the immune response during the induction and prevention of type 1 diabetes and how checkpoint inhibition impacts disease progression. Other duties as assigned. (15%) Qualifications Required Qualifications: Ph.D and B.S. or B.A. in biological sciences, related field, with 1 or more years of research training Five years Immunology research experience. This position requires the following techniques: Perform independent research projects as assigned by the PI or Scientific lab manager or Research lab manager. Perform independent research to evaluate the mechanism of action in murine cancer models with and without checkpoint inhibitors and monoclonal antibodies. Perform independent research evaluating antigen-specific T cell differentiation and expansion. Perform independent ELISA experiments on new mAbs targeting T cell receptors. Perform independent scientific calculations and statistical analysis using Prism or other related software. Perform independent Multicolor flow cytometry experimental set up and data acquisition. Analyze flow cytometry data using Flowjo or similar software independently. Write protocols performed, standard operating procedures, and prepare reports as assigned. Mouse colony management (SPF) and records keeping including data entry of genotyping results into large database records of colony using MS excel. Mous handling (SPF) Mouse breeding, weaning, and sample collection. (Mouse tail DNA isolation). Mouse genotyping ELiSPOT analysis Regulatory T cell cultures and inhibition assay for Treg impact on proliferation. Mouse tissue collection, including pancreas, lymph nodes, spleen, duodenum, jejunum, ileum, cecum. Mouse facial vein bleeding for blood collection Mouse islet cell isolation and transplantation Mouse injections (tail vein, SQ, ID, IM) Sterile cell culture in BSL2 conditions. Isolation of mononuclear cell enrichment from human PBMC samples. Preparing solutions. Work with biohazardous materials, chemicals, and hazardous wastes, as required for in vivo/in vitro/ex vivo operational procedures Immunohistochemistry Monoclonal antibody purification (mAbs) Support collaborative team-based research. Preferred Qualifications: The applicant must also be able to interact professionally with all faculty, students and staff who utilize the facility. Required computer skills include word-processing, spreadsheets, database and presentation, preferably with Microsoft Office Software. Excellent organizational skills, communication, and attention to detail is also required. Other preferred skills include Non-obese diabetic (NOD) mouse handling, monitoring and husbandry skills including diabetes monitoring and management, biological/cellular imaging, and human tissues, PCR troubleshooting, Western blot analysis, aseptic technique and assistance with surgical procedures is highly desirable. Benefits Working at the University At the University of Minnesota, you'll find a flexible work environment and supportive colleagues who are interested in lifelong learning. We prioritize work-life balance, allowing you to invest in the future of your career and in your life outside of work. The University also offers a comprehensive benefits package that includes: Competitive wages, paid holidays, and generous time off Continuous learning opportunities through professional training and degree-seeking programs supported by the Regents Tuition Benefit Program Low-cost medical, dental, and pharmacy plans Healthcare and dependent care flexible spending accounts University HSA contributions Disability and employer-paid life insurance Employee wellbeing program Excellent retirement plans with employer contribution Public Service Loan Forgiveness (PSLF) opportunity Financial counseling services Employee Assistance Program with eight sessions of counseling at no cost Please visit the Office of Human Resources website for more information regarding benefits. How To Apply Applications must be submitted online. To be considered for this position, please click the Apply button and follow the instructions. You will be given the opportunity to complete an online application for the position and attach a cover letter and resume. Additional documents may be attached after application by accessing your "My Job Applications" page and uploading documents in the "My Cover Letters and Attachments" section. To request an accommodation during the application process, please e-mail employ@umn.edu or call (612)... For full info follow application link. The University recognizes and values the importance of diversity and inclusion in enriching the employment experience of its employees and in supporting the academic mission. The University is committed to attracting and retaining employees with varying identities and backgrounds. The University of Minnesota provides equal access to and opportunity in its programs, facilities, and employment without regard to race, color, creed, religion, national origin, gender, age, marital status, disability, public assistance status, veteran status, sexual orientation, gender identity, or gender expression. To learn more about diversity at the U: http://diversity.umn.edu.