75154BRJob Posting Title:Research Assistant IDepartment:Medicine-Molecular MedicineAutoReqId:75154BRStatus:Full-TimeStandard Hours per Week:40 Job Posting Category:ResearchJob Posting Description:

We are seeking one laboratory technician at the level of Research Assistant I, who will be responsible for large-scale protein production in mammalian cells to support various projects in a structural biology laboratory.

Duties and responsibilities:

• Creating expression constructs for protein production; large-scale plasmid preparation.

• Maintaining mammalian cell lines.

• Testing expression constructs in mammalian cells (HEK293T, CHO cells, etc.).

• Scaling up the expression in mammalian cells to produce protein in milligram quantities

• Purifying protein by various chromatographic techniques following established protocols.

• Performing SDS-PAGE and western blots to monitor proteins at various stages of protein expression and purification.

• Maintaining inventory of cell culture supplies as well as contributing to general maintenance of the laboratory, equipment, and supplies.

Required Education, Experience and Skills:

• Candidates must have a bachelor's degree in the biological sciences.

• Experience in cell culture or protein expression and purification is a plus.

• Effective communication and organizational skills

• Ability to work in a team environment are necessary

Boston Children’s Hospital offers competitive compensation and unmatched benefits including flexible schedules, affordable health, vision and dental insurance, child care and student loan subsidies, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition and certain License and Certification Reimbursement, cell phone plan discounts and discounted rates on T-passes. Experience the benefits of passion and teamwork.

#LI-Onsite

Office/Site Location:BostonRegular, Temporary, Per Diem:Regular Remote Eligibility :Onsite Only