Express, purify, and characterize full-length and deletion constructs of group II intron and other non-LTR retroelement reverse transcriptase and related proteins for structural analysis and biotechnological applications using Fast Protein Liquid Chromatography FPLC and other methods. Characterize wild-type and mutant proteins biochemically via enzymatic assays and determine conditions for obtaining diffraction quality crystals and/or for cryo-electron microscopy analysis of proteins and protein/nucleic acid complexes. Carry out biochemical and biophysical assays. Obtain structures of reverse transcriptase and related enzymes and their nucleic acid-protein complexes by X-ray diffraction and/or cryo-EM structures.Write manuscripts and assist in the preparation of reports, abstracts, manuscripts and proposals. Make presentations at lab meetings. Attend lab meetings and other meetings. Order chemicals and supplies specific to current project. Provide instruction, assistance and mentoring to graduate students.